
Page 4 of 4
Cazanetal. Acta Vet Scand (2020) 62:42
Acknowledgements
The authors would like to thank the kennel personnel for all their help during
the study.
Authors’ contributions
MOD and AMI designed the study. CDC, IAM and GD participated in the field-
work. CDC, CM, EB, AMI and MOD serologically and molecularly identified the
pathogen species. AMI and MOD performed the data analysis. CDC drafted
the original manuscript. AMI, IAM, CM, EB, GD and MOD critically revised the
manuscript for important intellectual content. All authors read and approved
the final manuscript.
Funding
MOD was the grant recipient of CNCS-UEFISCDI Grant Agency Romania,
research grants PD38/2018 and TE299/2015. The work of MOD was supported
by the research grants PD38/2018 and TE299/2015. The work of CDC, AMI, IAM
and GD was supported by the research grant TE 299/2015. The publication
was supported by funds from the National Research Development Projects to
finance excellence (PFE)-37/2018–2020 granted by the Romanian Ministry of
Research and Innovation.
Availability of data and materials
All data generated or analysed during this study are included in this published
article.
Ethics approval and consent to participate
Not applicable.
Consent for publication
Not applicable.
Competing interests
The authors declare that they have no competing interests.
Author details
1
Department of Parasitology and Parasitic Diseases, University of Agricul-
tural Sciences and Veterinary Medicine of Cluj-Napoca, Calea Mănăștur 3-5,
400372 Cluj-Napoca, Romania.
2
CDS-9, “Regele Mihai I al Romaniei” Life
Science Institute, University of Agricultural Sciences and Veterinary Medicine
of Cluj-Napoca, Calea Mănăștur 3-5, 400372 Cluj-Napoca, Romania.
3
Depart-
ment of Microbiology, Immunology and Epidemiology, University of Agri-
cultural Sciences and Veterinary Medicine of Cluj-Napoca, Calea Mănăștur
3-5, 400372 Cluj-Napoca, Romania.
4
Department of Animal Health, Faculty
of Veterinary Science, Regional Campus of International Excellence ‘Campus
Mare Nostrum’, University of Murcia, 30100 Murcia, Spain.
Received: 31 March 2020 Accepted: 28 July 2020
References
1. Day MJ. One health: the importance of companion animal vector-borne
diseases. Parasites Vectors. 2011;4:49.
2. Otranto O, Dantas-Torres F, Breitschwerdt EB. Managing canine vectorborne
diseases of zoonotic concern: part one. Trends Parasitol. 2009;25:157–63.
3. Day MJ. The immunopathology of canine vector-borne diseases. Parasites
Vectors. 2011;4:48.
4. Mihalca AD, Cazan CD, Sulesco T, Dumitrache MO. A historical review on
vector distribution and epidemiology of human and animal leishmanioses
in Eastern Europe. Res Vet Sci. 2019;123:185–91.
5. Mircean V, Dumitrache MO, Mircean M, Bolfa P, Györke A, Mihalca AD.
Autochthonous canine leishmaniasis in Romania: neglected or (re)emerg-
ing? Parasites Vectors. 2014;7:135.
6. Dumitrache MO, Nachum-Biala Y, Gilad M, Mircean V, Cazan CD, Mihalca
AD, et al. The quest for canine leishmaniasis in Romania: the presence of an
autochthonous focus with subclinical infections in an area where disease
occurred. Parasites Vectors. 2016;9:297.
7. Cîmpan AA, Diakou A, Papadopoulos E, Miron LD. Serological study of
exposure to Leishmania in dogs living in shelters, in South-East Romania.
Rev Rom Med Vet. 2019;29:54–8.
8. Sauda F, Malandrucco L, Macrì G, Scarpulla M, de Liberato C, Terracciano G,
et al. Leishmania infantum, Dirofilaria spp. and other endoparasite infections
in kennel dogs in Central Italy. Parasite. 2018;25:2.
9. Cardoso L, Mendão C, Madeira de Carvalho L. Prevalence of Dirofilaria
immitis, Ehrlichia canis, Borrelia burgdorferi sensu lato, Anaplasma spp. and
Leishmania infantum in apparently healthy and CVBD-suspect dogs in
Portugal-a national serological study. Parasites Vectors. 2012;5:62.
10. Francino O, Altet L, Sanchez-Robert E, Rodriguez A, Solano-Gallego L,
Alberola J, et al. Advantages of real-time PCR assay for diagnosis and moni-
toring of canine leishmaniosis. Vet Parasitol. 2006;137:214–21.
11. Solano-Gallego L, Miró G, Koutinas A, Cardoso L, Pennisi MG, Ferrer L, et al.
LeishVet guidelines for the practical management of canine leishmaniosis.
Parasites Vectors. 2011;4:86.
12. Aoun O, Mary C, Roqueplo C, Marié JL, Terrier O, Levieuge A, et al. Canine
leishmaniasis in south-east of France: screening of Leishmania infantum
antibodies (western blotting, ELISA) and parasitaemia levels by PCR quantifi-
cation. Vet Parasitol. 2009;166:27–31.
13. Moshfe A, Mohebali M, Edrissian G, Zarei Z, Akhoundi B, Kazemi B, et al.
Canine visceral leishmaniasis: asymptomatic infected dogs as a source of L.
infantum infection. Acta Trop. 2009;112:101–5.
14. Cabezón O, Millán J, Gomis M, Dubey JP, Ferroglio E, Almeria S. Kennel
dogs as sentinels of Leishmania infantum, Toxoplasma gondii, and Neospora
caninum in Majorca Island, Spain. Parasitol Res. 2010;107:1505–8.
15. Wang JY, Ha Y, Gao CH, Wang Y, Yang YT, Chen HT. The prevalence of canine
Leishmania infantum infection in western China detected by PCR and
serological tests. Parasites Vectors. 2011;4:69.
16. Fakhar M, Motazedian MH, Asgari Q, Kalantari M. Asymptomatic domestic
dogs are carriers of Leishmania infantum: possible reservoirs host for human
visceral leishmaniasis in southern Iran. Comp Clin Pathol. 2012;21:801–7.
17. Pantchev N, Schnyder M, Vrhovec MG, Schaper R, Tsachev I. Current surveys
of the seroprevalence of Borrelia burgdorferi, Ehrlichia canis, Anaplasma phago-
cytophilum, Leishmania infantum, Babesia canis, Angiostrongylus vasorum and
Dirofilaria immitis in dogs in Bulgaria. Parasitol Res. 2015;114:117–30.
18. Kalmár Z, Mihalca AD, Dumitrache MO, Gherman CM, Magdaş C, Mircean
V, et al. Geographical distribution and prevalence of Borrelia burgdorferi
genospecies in questing Ixodes ricinus from Romania: a countrywide study.
Ticks Tick-Borne Dis. 2013;4:403–8.
19. Răileanu C, Moutailler S, Pavel I, Porea D, Mihalca AD, Savuta G, et al. Borrelia
diversity and co-infection with other tick borne pathogens in ticks. Front
Cell Infect Microbiol. 2017;7:36.
20. Mircean V, Dumitrache MO, Györke A, Pantchev N, Jodies R, Mihalca AD,
et al. Seroprevalence and geographic distribution of Dirofilaria immitis and
tick borne infections (Anaplasma phagocytophilum, Borrelia burgdorferi
sensu lato, and Ehrlichia canis) in dogs from Romania. Vector Borne Zoonotic
Dis. 2012;12:595–604.
21. Egyed L, Élő P, Sréter-Lancz Z, Széll Z, Balogh Z, Sréter T. Seasonal activity and
tick-borne pathogen infection rates of Ixodes ricinus ticks in Hungary. Ticks
Tick Borne Dis. 2012;3:90–4.
22. Matei IA, Kalmár Z, Magdaș C, Magdaș V, Toriay H, Dumitrache MO, et al.
Anaplasma phagocytophilum in questing Ixodes ricinus ticks from Romania.
Ticks Tick Borne Dis. 2014;6:408–13.
23. Ionică AM, Matei IA, Mircean V, Dumitrache MO, D’Amico G, Győrke A,
et al. Current surveys on the prevalence and distribution of Dirofilaria
spp. and Acanthocheilonema reconditum infections in dogs in Romania.
Parasitol Res. 2015;114:975–82.
24. Solano-Gallego L, Morell P, Arboix M, Alberola J, Ferrer L. Prevalence of Leishma-
nia infantum infection in dogs living in an area of canine leishmaniasis endemic-
ity using PCR on several tissues and serology. J Clin Microbiol. 2001;39:560–3.
25. Simonato G, Frangipane di Regalbono A, Cassini R, Traversa D, Beraldo P, Tes-
sarin C, et al. Copromicroscopic and molecular investigations on intestinal
parasites in kenneled dogs. Parasitol Res. 2015;114:1963–70.
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